ribU [2019-06-06 11:39:07]

riboflavin [SW|ECF transporter], S protein

locus: BSU23050

pI: 10.12

mw: 20.41 kDa

protein length: 190 aa

gene length: 573 bp

function: riboflavin uptake

product: S protein of riboflavin [SW|ECF transporter]

essential: no

synonyms: ypaA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.2|The substrate-specific S components of the ECF transporters]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.2|Biosynthesis/ acquisition of riboflavin/ FAD]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,410,017 2,410,589

The protein

Structure

• [PDB|3P5N] (RibU from ''Staphylococcus aureus'')

[SW|Localization]

• membrane

Expression and Regulation

Operons

• genes: [gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]
  description: [Pubmed|21815947]

  regulatory mechanism

  • [regulon|FMN-box|FMN-box]: transcription termination, via [protein|C245476CE35028167510F67153F392D91447553F|MgtE riboswitch], sequestration of the Shine-Dalgarno sequence in the presence of FMN or FMNH₂, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|RibR], in [regulon|FMN-box|FMN-box]

  regulation

  • expressed in the absence of FMN ([protein|search|FMN-box]) [Pubmed|17693491]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-A478 (ypaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/478 NBRP B. subtilis, Japan]
• BKE23050 ([gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC, downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT
• BKK23050 ([gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC, downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT

References

Reviews

• 20497229,22574898,24362466

Original publications

• 12456892,20972419,17693491,21815947,24356467,24755604,25438558,26494285,27312125